Some of the associations between the HLA genes and diseases have been known for decades. However, these results mainly came from limited disease groups and are often based on Caucasian. In order to explore the association between HLA genes and disease in larger real-world data in Taiwan, we analyzed the Chang Gung Research Database, the largest healthcare system in Taiwan, combined with Taiwan Biobank in this study. Patients received human leukocyte antigen genotype tests between 2001 and 2019 in the Linkou branch of Chang Gung Memorial Hospital. The HLA tests were included HLA-A/B/C/DR sequence-specific oligonucleotide test(SSO) test, HLA-A/B/C/DR sequencing-based typing(SBT) test, HLA-B*15:02, and HLA-B27 flow cytometry test. The patient had to be followed up for more than 1 year at our outpatient department. Besides, the normal population of HLA genotype information in Taiwan was acquired from Taiwan Biobank. There are a total of 22752 patients enrolled in our study from the Chang Gung Research Database. These patients had HLA genotyping between 2001 and 2019, also followed up more than 1 year at our outpatient department. Besides the real-world data acquired from the Chang Gung Research Database, we acquired the HLA typing data containing 1103 volunteers registered in Taiwan Biobank. Through comparison between two groups, our result revealed the compatible association between disease and genotype according to previous research. Our work also showed some limitations from analyzing through previous real-world clinical data. There have been some genotype methodology shifts during the past decade and the resolution of HLA genotypes has also changed, which also affects our analysis. Besides, most of the patients with HLA genotypes in our hospital are limited to certain clinical scenarios, such as hematopoietic stem cell transplantation, solid organ transplantation. In conclusion, we conducted the association investigation study between HLA genotypes and disease with our established Chang Gung Research Database. Besides, using the Taiwan Biobank data can adjust certain biases from hospital central data and provide baseline reference, which is not easily acquired from most hospital researchers by themselves.